They are using a kind of next generation sequencing technology called targeted sequencing to sequence up to 100 genes in several individuals simultaneously. This project is ongoing, and they’re still figuring out the limits of how many individuals they can run at once, but it’s probably around 25-30. They plan to use the data to determine the genetic differences that exist between susceptible and resistant individuals and aren’t due to other factors. They expect to find differences in some genes that have not been previously well-characterized, and plan to develop diagnostic assays for these genes so that the CDC or other agencies tasked with mosquito control can test their mosquitoes for resistance. These are useful tools for local vector control programs studying the Culex pipiens mosquitoes.
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